Ion Exchange Chromatography of Intact Brain Phosphoinositides on Diethylaminoethyl Cellulose by Gradient Salt Elution in a Mixed Solvent System
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Chromatography of Myoglobin on Diethylaminoethyl Cellulose Columns*
Since the initial description (2, 3) of procedures for the use of cellulose ion exchange columns for the chromatography of proteins, a number of such systems have been developed for specific proteins, including the heme proteins. Thus, Gutter et al. (4) have described the use of carboxymethyl cellulose in a study of various hemoglobins, Rumen (5) has utilized carboxymethyl cellulose for the ide...
متن کاملFractionation of soluble ribonucleic acid on diethylaminoethyl Sephadex with a pH and salt gradient for elution.
A new method for the chromatographic fractionation of soluble ribonucleic acid (sRNA) is described. A gradient of increasing salt and pH is employed to elute sRNA from diethylaminoethyl Sephadex. The resolution of sRNA species for 10 different amino acids obtained with this method is presented. There is evidence that resolution is dependent upon a change in sRNA from a partially denatured state...
متن کاملFractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose.
Wilson, Dwight E. (Rensselaer Polytechnic Institute, Troy, N.Y.). Fractionation of Newcastle disease virus by chromatography on diethylaminoethyl cellulose. J. Bacteriol. 84:295-301. 1962.-The L. Kansas and NK strains of Newcastle disease virus were chromatographed on diethylaminoethyl (DEAE) cellulose ion-exchange columns. L. Kansas virus eluted from DEAE columns showed one peak of hemagglutin...
متن کاملThe fractionation of phosphate esters on ion-exchange resin by a new system of pH-gradient elution.
Chargaff, E. & Cohen, S. S. (1939). J. biol. Chem. 129, 619. Dawson, R. M. C. & Elliott, W. H. (1959). In Data for Biochemical Re8earch, p. 192. Ed. by Dawson, R. M. C., Elliott, D. C., Elliott, W. H. & Jones, K. M. Oxford: Clarendon Press. Fairbairn, D. (1945). J. biol. Chem. 157, 633. Folch, J., Lees, M. & Sloane-Stanley, G. H. (1957). J. biol. Chem. 226, 497. Gomori, G. (1955). In Methods in...
متن کاملLarge-Scale Gradient Elution Chromatography
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1964
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)91323-2